Human PGC1 alpha antibody | NKMAX ATGA0466 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human PGC1 alpha antibody

catalog :

ATGA0466

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT25C8

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human PGC1 alpha antibody

Catalog number (SKU) :

ATGA0466

NCBI :

NP_037393

Description :

PPARGC1A is transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. This protein can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis and plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Also, induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner and involved in the integration of the circadian rhythms and energy metabolism. They required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK.

Alternative Names :

Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, PGC-1-alpha, PPAR-gamma coactivator 1-alpha, PPARGC-1-alpha, Ligand effect modulator 6, PPARGC1A, LEM6, PGC1, PGC1A, PPARGC1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Lui Z., et al. (2016) Sci Rep. 6: 21382.;; Li R., et al. (2016) Med Sci Monit. 22: 3229-3237.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human PPARGC1A (300-540aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of PPARGC1A in HeLa cells. The cell was stained with ATGA0466 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPARGC1A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: HeLa cell lysate ;;Lane 2.: MCF7 cell lysate ;;Lane 3.: HepG2 cell lysate ;;Lane 4.: 293T cell lysate ;;Lane 5.: Mouse brain tissue lysate

Reacts With :

Human

Clone Number :

AT25C8

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of PPARGC1A in HeLa cells. The cell was stained with ATGA0466 at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q9UBK2

Internal Search Terms :

PGC1 a,ATGA0466-10ug,ATGA0466-20ug,ATGA0466-50ug,ATGA0466-100ug,ATGA0466-250ug,ATGA0466-500ug,ATGA0466-1mg,ATGA0466-10,ATGA0466-20,ATGA0466-50,ATGA0466-100,ATGA0466-250,ATGA0466-500,ATGA0466-1

more info or order :

NKMAX product webpage