Human NM23-H1/NME1 antibody | NKMAX ATGA0461 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human NM23-H1/NME1 antibody

catalog :

ATGA0461

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT5F4

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human NM23-H1/NME1 antibody

Catalog number (SKU) :

ATGA0461

NCBI :

NP_000260

Description :

Non-metastatic cells 1 (NME1), also known as NM23-H1, originally identified as a candidate metastasis suppressor gene. NME1 is expressed in different tumor types where their levels have been alternatively associated with reduced or increased metastatic potential. Reductions in NME1 expression have been significantly associated with aggressive behavior in melanoma, breast, colon, and gastric carcinomas. On the contrary, high levels of NME1 gene expression are noted in the advanced stage of thyroid carcinomas.

Alternative Names :

NME/NM23 nucleoside diphosphate kinase 1, Nucleoside diphosphate kinase A, Granzyme A-activated Dnase, GAAD, Metastasis inhibition factor nm23, NM23-H1, Tumor metastatic process-associated protein, NDK A, NDP kinase A, NDPKA, NM23

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Tee YT., et al. (2006) Taiwan J Obstet Gynecol. 45(2): 107-13.;;Negroni A., et al. (2000) Cell Death Differ. 7(9): 843-50.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human NME1 (1-152aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of NME1 in HeLa cells. The cell was stained with ATGA0461 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).ICC/IF analysis of NME1 in A549 cells. The cell was stained with ATGA0461 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{ATGA0461-IF2.jpg}

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1 : HeLa cell lysate;;Lane 2 : A549 cell lysate;;Lane 3 : Jurkat cell lysate;;Lane 4 : HepG2 cell lysate;;Lane 5 : MCF7 cell lysate;;Lane 6 : PC3 cell lysate The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1 : Recombinant Human NME1 protein;;Lane 2 : Recombinant Human NME2 protein;;Lane 3 : Recombinant Human NME3 protein;;Lane 4 : Recombinant Human NME4 protein{ATGA0461-WB2.jpg}

Reacts With :

Human

Clone Number :

AT5F4

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P15531

Internal Search Terms :

NM23-H1,ATGA0461-10ug,ATGA0461-20ug,ATGA0461-50ug,ATGA0461-100ug,ATGA0461-250ug,ATGA0461-500ug,ATGA0461-1mg,ATGA0461-10,ATGA0461-20,ATGA0461-50,ATGA0461-100,ATGA0461-250,ATGA0461-500,ATGA0461-1

more info or order :

NKMAX product webpage