Human Leptin/OB antibody | NKMAX ATGA0460 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Leptin/OB antibody

catalog :

ATGA0460

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT7E10

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Leptin/OB antibody

Catalog number (SKU) :

ATGA0460

NCBI :

NP_000221

Description :

Human leptin is a 16-kDa nonglycosylated hormone that is produced in mature adipocytes. Leptin acts primarily in the hypothalamus to reduce food intake and body weight. In ob/ob mice, the gene encoding leptin is mutated, resulting in morbid obesity and associated abnormalities, including hyperphagia, hypothermia, diabetes and infertility. The function of leptin in regulating appetite and metabolism, as well as the possibility of using leptin as a therapeutic agent, are currently under intense investigation.

Alternative Names :

LEP, OB, OBS, Leptin, Obesity factor, Obese protein, Leptin, Leptin Murine Obesity Homolog, Leptin Precursor Obesity Factor, Obesity, Obesity homolog mouse, Obesity Murine Homolog Leptin

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Anini Y., et al. (2003) Diabetes. 52(2): 252-259.;; Jeong KJ., et al. (1999) Appl Environ Microbiol. 65(7): 3027-32.;; Hyogo H., et al. (2002) J. Biol. Chem. 277(37): 34117-34124.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human Leptin (22-167aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of Leptin in HeLa cells. The cell was stained with ATGA0460 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Leptin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;; Lane 2.: Leptin transfected 293T cell lysate

Reacts With :

Human

Clone Number :

AT7E10

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P41159

Internal Search Terms :

Leptin, OB,ATGA0460-10ug,ATGA0460-20ug,ATGA0460-50ug,ATGA0460-100ug,ATGA0460-250ug,ATGA0460-500ug,ATGA0460-1mg,ATGA0460-10,ATGA0460-20,ATGA0460-50,ATGA0460-100,ATGA0460-250,ATGA0460-500,ATGA0460-1

more info or order :

NKMAX product webpage