Human RKIP/PEBP1 antibody | NKMAX ATGA0456 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human RKIP/PEBP1 antibody

catalog :

ATGA0456

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT4B11

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human RKIP/PEBP1 antibody

Catalog number (SKU) :

ATGA0456

NCBI :

NP_002558

Description :

Phosphatidylethanolamine binding protein 1 (PEBP1), also known as Raf kinase inhibitor protein (RKIP), is a member of the phosphatidylethanolamine-binding protein family and a serine protease inhibitor which inhibits thrombin, neuropsin. PEBP1 plays a pivotal modulatory role in several protein kinase signaling cascades. Protein kinase C (PKC) phosphorylates PEBP1, resulting in release of Raf-1 and activation of MEK and ERK. Expressed in many tissues, PEBP1 is implicated in the regulation of such physiological processes as membrane biosynthesis, spermatogenesis, neural development, and metastasis suppression.

Alternative Names :

Phosphatidylethanolamine binding protein 1, PBP, Prostatic binding protein, RKIP, HCNP, HCNPpp, PEBP, Raf kinase inhibitory protein, Hippocampal cholinergic neurostimulating peptide

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Keller ET., et al. (2004) Biochem Pharmacol. 68(6): 1049-53. ;; Park S., et al. (2005) Oncogene. 24(21): 3535-40.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human PEBP1 (1-187aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of PEBP1 in HeLa cells line, monoclonal anti-human PEBP1 antibody (1:100) stained with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PEBP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1 : HeLa cell lysate;;Lane 2 : SKOV3 cell lysate;;Lane 3 : A549 cell lysate;;Lane 4 : HepG2 cell lysate;;Lane 5 : MCF7 cell lysate;;Lane 6 : K562 cell lysate;;Lane 7 : LnCap cell lysate;;Lane 8 : Ramos cell lysate

Reacts With :

Human

Clone Number :

AT4B11

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Cell Cycle

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P30086

Internal Search Terms :

RKIP, PEBP1,ATGA0456-10ug,ATGA0456-20ug,ATGA0456-50ug,ATGA0456-100ug,ATGA0456-250ug,ATGA0456-500ug,ATGA0456-1mg,ATGA0456-10,ATGA0456-20,ATGA0456-50,ATGA0456-100,ATGA0456-250,ATGA0456-500,ATGA0456-1

more info or order :

NKMAX product webpage