Human BPGM antibody | NKMAX ATGA0455 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human BPGM antibody

catalog :

ATGA0455

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2E11

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human BPGM antibody

Catalog number (SKU) :

ATGA0455

NCBI :

NP_001715

Description :

Bisphosphoglycerate mutase (BPGM) is an enzyme unique to erythrocytes and placental cells. This protein plays a major role in regulating hemoglobin oxygen affinity as a consequence of controlling 2, 3-BPG concentration. It is responsible for the catalytic synthesis of 2, 3-Bisphosphoglycerate (2, 3-BPG) from 1, 3-BPG. BPGM also has a mutase and a phosphatase function, but these are much less active.

Alternative Names :

Bisphosphoglycerate mutase, BPG-dependent PGAM, Bisphosphoglycerate mutase 2,3-bisphosphoglycerate mutase, erythrocyte, 2,3-bisphosphoglycerate mutase, 2,3-bisphosphoglycerate synthase, Ab2 098, AI323730 AL022789, BPG dependent PGAM, C86192.

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Pritlove DC., et al. (2006) Placenta. 27(8): 924-7. ;; Wang Y., et al. (2006) J. Biol. Chem. 281(51): 39642-8.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human BPGM (1-259aa) purified from E. coli.

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of BPGM in HeLa cells. The cell was stained with ATGA0445 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates(10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BPGM antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: 293T cell lysate;; Lane 2.: BPGM transfected 293T cell lysate

Reacts With :

Human

Clone Number :

AT2E11

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of BPGM in HeLa cells. The cell was stained with ATGA0455 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P07738

Internal Search Terms :

BPGM, Bisphosphoglycerate mutase, BPG-dependent PGAM, Bisphosphoglycerate mutase 2,3-bisphosphoglycerate mutase, erythrocyte, 2,3-bisphosphoglycerate mutase, 2,3-bisphosphoglycerate synthase, Ab2 098, AI323730 AL022789, BPG dependent PGAM, C86192.,ATGA0455-10ug,ATGA0455-20ug,ATGA0455-50ug,ATGA0455-100ug,ATGA0455-250ug,ATGA0455-500ug,ATGA0455-1mg,ATGA0455-10,ATGA0455-20,ATGA0455-50,ATGA0455-100,ATGA0455-250,ATGA0455-500,ATGA0455-1

more info or order :

NKMAX product webpage