Human HO-1/HMOX1/HSP32 antibody | NKMAX ATGA0454 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human HO-1/HMOX1/HSP32 antibody

catalog :

ATGA0454

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1D6

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human HO-1/HMOX1/HSP32 antibody

Catalog number (SKU) :

ATGA0454

NCBI :

NP_002124

Description :

Heme oxygenase 1 belongs to the heme oxygenase family and is an essential enzyme in heme catabolism. It cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Also this protein is known to play an important role in the regulation of cardiovascular function and its adaptive response to a variety of stressors.

Alternative Names :

HO-1, Heat shock protein 32, HSP32, bK286B10, D8Wsu38e, Heme oxygenase (decycling) 1, Heme oxygenase 1, Hemox, Hmox, HMOX 1, HMOX1, HO, HO 1, HO1.

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Soares MP., et al. (2001) Immunol Rev.;; Vareille M., et al. (2008) J Immunol. 180(8): 5720-6.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human Heme oxygenase1 (1-266aa) purified from E. coli

Applications :

ELISA,WB,FACS,ICC/IF

IF Comment And Pic :

ICC/IF analysis of HMOX in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HMOX antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) {ATGA0454-IF1b.jpg}ICC/IF analysis of HMOX in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HMOX antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) {ATGA0454-IF2a.jpg} {ATGA0454-IF2b.jpg}

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HMOX antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: HeLa cell lysate ;;Lane 2.: A549 cell lysate ;;Lane 3.: 293T cell lysate ;;Lane 4.: HepG2 cell lysate ;;Lane 5.: TF-1 cell lysate

Reacts With :

Human

Clone Number :

AT1D6

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of HMOX in HeLa cells. The cell was stained with ATGA0454 at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Neuroscience

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P09601

Internal Search Terms :

HO1, HMOX1, HSP32, HO-1, Heat shock protein 32, HSP32, bK286B10, D8Wsu38e, Heme oxygenase (decycling) 1, Heme oxygenase 1, Hemox, Hmox, HMOX 1, HMOX1, HO, HO 1, HO1.,ATGA0454-10ug,ATGA0454-20ug,ATGA0454-50ug,ATGA0454-100ug,ATGA0454-250ug,ATGA0454-500ug,ATGA0454-1mg,ATGA0454-10,ATGA0454-20,ATGA0454-50,ATGA0454-100,ATGA0454-250,ATGA0454-500,ATGA0454-1

more info or order :

NKMAX product webpage