Human Vimentin antibody | NKMAX ATGA0453 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Vimentin antibody

catalog :

ATGA0453

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT5H8

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Vimentin antibody

Catalog number (SKU) :

ATGA0453

NCBI :

NP_003371

Description :

Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all metazoan cells as well as bacteria. IF, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastaticprogression.

Alternative Names :

Vimentin, epididymis luminal protein 113, HEL113, CTRCT30

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

John E. Eriksson.,et al. (2009) J Clin Invest. 119(7): 1763-1771. ;; Cabeen MT.,et al. (2010) Annu Rev Genet. 44: 365-392.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human VIM (1-466aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of VIM in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human VIM antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). {ATGA0453-IF1b.jpg}

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VIM antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1 : Jurkat cell lysate ;;Lane 2 : K562 cell lysate ;;Lane 3 : 293T cell lysate ;;Lane 4 : A549 cell lysate ;;Lane 5 : SK-OV-3 cell lysate ;;Lane 6 : HeLa cell lysate

Reacts With :

Human

Clone Number :

AT5H8

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Neuroscience

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P08670

Internal Search Terms :

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more info or order :

NKMAX product webpage