Human GMF-beta/GMFB antibody | NKMAX ATGA0452 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human GMF-beta/GMFB antibody

catalog :

ATGA0452

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT44D8

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human GMF-beta/GMFB antibody

Catalog number (SKU) :

ATGA0452

NCBI :

NP_004115

Description :

GMFB, also known as GMF, belongs to the GMF subfamily of the larger actin-binding protein ADF family. This protein, which is phosphorylated following phorbol ester stimulation, is important for the nervous system. It causes brain cell differentiation, stimulates neural regeneration and inhibits tumor cell proliferation. Overexpression of GMFB in astrocytes has been shown to enhance brain-derived neurotrohic factor (BDNF) production. GMFB expression is increased by exercise, and the protein is crucial for exercise-induction of BDNF.

Alternative Names :

Glia maturation factor beta, GMF, GMF beta, Glia maturation factor beta

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Li YL., et al. (2010) Eur J Cancer. 46(11): 2104-18. ;; Wada T., et al. (2009) Int J Oncol. 35(5): 973-6.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human GMFB (1-142aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of GMFB in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GMFB antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

WB Comment and Pic :

The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GMFB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: PC3 cell lysate The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GMFB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: 293T cell lysate ;;Lane 2.: GMFB transfected 293T cell lysate {ATGA0452-WB2.jpg}

Reacts With :

Human

Clone Number :

AT44D8

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Neuroscience

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P60983

Internal Search Terms :

GMF-b, GMFB, Glia maturation factor beta, GMF, GMF beta, Glia maturation factor beta,ATGA0452-10ug,ATGA0452-20ug,ATGA0452-50ug,ATGA0452-100ug,ATGA0452-250ug,ATGA0452-500ug,ATGA0452-1mg,ATGA0452-10,ATGA0452-20,ATGA0452-50,ATGA0452-100,ATGA0452-250,ATGA0452-500,ATGA0452-1

more info or order :

NKMAX product webpage