Human Aldehyde dehydrogenase 2/ALDH2 antibody | NKMAX ATGA0450 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Aldehyde dehydrogenase 2/ALDH2 antibody

catalog :

ATGA0450

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2C10

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Aldehyde dehydrogenase 2/ALDH2 antibody

Catalog number (SKU) :

ATGA0450

NCBI :

NP_000681

Description :

ALDH2 (Aldehyde dehydrogenase 2 family) belongs to the aldehyde dehydrogenase family which catalyze the chemical transformation from acetaldehyde to acetic acid and is the second enzyme of the major oxidative pathway of alcohol metabolism. There are two major liver isoforms of this enzyme, cytosolic and mitochondrial, and they can be also distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations.

Alternative Names :

Aldehyde dehydrogenase 2 family member, Aldehyde dehydrogenase mitochondrial, ALDH class 2, ALDH-E2, ALDHI, ALDM

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Crabb DW., et al. (1989) J Clin Invest. 83(1): 314-6. ;; Feng Liu., et al. (2002) Plant Physiol. 130(4): 1657-1674.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human ALDH2 (18-517aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of ALDH2 in A549 cells line, stained with monoclonal anti-human ALDH2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

WB Comment and Pic :

The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDH2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1. : HepG2 cell lysate ;;Lane 2. : Mouse liver tissue lysate ;;Lane 3. : Mouse lung tissue lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDH2 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1. : 293T cell lysate ;;Lane 2. : ALDH2 transfected 293T cell lysate {ATGA0450-WB2.jpg}

Reacts With :

Human

Clone Number :

AT2C10

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Redox Proteins

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P05091

Internal Search Terms :

ALDH2, Aldehyde dehydrogenase mitochondrial, ALDH-E2, ALDHI, ALDM, Aldehyde dehydrogenase, Mitochondrial Acetaldehyde dehydrogenase 2, Aldehyde dehydrogenase 2 family, Aldehyde dehydrogenase 2 family (mitochondrial), ALDHE2, ALDH 2, ALDH class 2, ALDH E2, Liver mitochondrial ALDH, MGC1806, Mitochondrial aldehyde dehydrogenase 2, Nucleus encoded mitochondrial aldehyde dehydrogenase 2, ATGA0450-10ug, ATGA0450-20ug, ATGA0450-50ug, ATGA0450-100ug, ATGA0450-250ug, ATGA0450-500ug, ATGA0450-1mg, ATGA0450-010, ATGA0450-020, ATGA0450-050, ATGA0450-100, ATGA0450-250, ATGA0450-500, ATGA0450-01M

more info or order :

NKMAX product webpage