Human PPME1 antibody | NKMAX ATGA0449 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human PPME1 antibody

catalog :

ATGA0449

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT29G3

reactivity :

human

application :

western blot, ELISA

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human PPME1 antibody

Catalog number (SKU) :

ATGA0449

NCBI :

NP_057231

Description :

PPME1, also known protein phosphatase methylesterase-1 (PME1), catalyzes the demethylation and inactivation of protein phosphatase (PP2A), which is a multimeric phosphoserine/ threonine protein phosphatase associated with growth inhibition and cell cycle arrest. It can demethylate PP2A catalytic subunit in vitro and okadaic acid treatment is capable of inhibiting this reaction. It is conserved from yeast to human and contains a motif found in lipases having a catalytic triad activated serine as their active site nucleophile.

Alternative Names :

Protein phosphatase methylesterase 1, FLJ22226, PME-1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Ogris E., et al. (1999) J. Biol. Chem. 274: 14382-14391.;; Gagnon S.N., et al. (2002) Biochem. J. 368: 263-271.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human PPME1 (1-386aa) purified from E. coli

Applications :

ELISA, WB

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPME1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: HeLa cell lysate ;;Lane 2.: K562 cell lysate ;;Lane 3.: A549 cell lysate

Reacts With :

Human

Clone Number :

AT29G3

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q9Y570

Internal Search Terms :

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more info or order :

NKMAX product webpage