Human AK1 antibody | NKMAX ATGA0447 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human AK1 antibody

catalog :

ATGA0447

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT7E9

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human AK1 antibody

Catalog number (SKU) :

ATGA0447

NCBI :

NP_000467

Description :

AK1 is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. This protein is found in the cytosol of skeletal muscle, brain and erythrocytes. It is a small ubiquitous enzyme which is essential for maintenance and cell growth. Defects in AK1 are the cause of a form of hemolytic anemia.

Alternative Names :

Adenylate kinase isoenzyme1, ATP-AMP transphosphorylase 1, Myokinase, Adenylate kinase 1, ATP:AMP phosphotransferase, Adenylate monophosphate kinase

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Terzic A., et al. (2007) J Biol Chem. 282(43): 31366-72.;; Morelli A., et al. (2007) Curr Eye Res. 32(3): 249-57.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human AK1 (1-194aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of AK1 in HeLa cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).{ATGA0447-IF1b.jpg}ICC/IF analysis of AK1 in Hep3B cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).{ATGA0447-IF2a.jpg}{ATGA0447-IF2b.jpg}ICC/IF analysis of AK1 in A549 cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). {ATGA0447-IF3a.jpg}{ATGA0447-IF3b.jpg}

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: HeLa cell lysate ;;Lane 2.: U87-MG cell lysate ;;Lane 3.: K562 cell lysate ;;Lane 4.: 293T cell lysate ;;Lane 5.: HepG2 cell lysate ;;Lane 6.: A549 cell lysate ;;Lane 7.: MCF7cell lysate ;;Lane 8.: SK-OV-3 cell lysate ;;Lane 9.: PC3 cell lysate

Reacts With :

Human

Clone Number :

AT7E9

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P00568

Internal Search Terms :

AK1, Adenylate kinase isoenzyme1, ATP-AMP transphosphorylase 1, Myokinase,ATGA0447-10ug,ATGA0447-20ug,ATGA0447-50ug,ATGA0447-100ug,ATGA0447-250ug,ATGA0447-500ug,ATGA0447-1mg,ATGA0447-10,ATGA0447-20,ATGA0447-50,ATGA0447-100,ATGA0447-250,ATGA0447-500,ATGA0447-1

more info or order :

NKMAX product webpage