Human PSMD11 antibody | NKMAX ATGA0446 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human PSMD11 antibody

catalog :

ATGA0446

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2C7

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human PSMD11 antibody

Catalog number (SKU) :

ATGA0446

NCBI :

NP_002806

Description :

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. PSMD11 is a member of the proteasome subunit S9 family that functions as a nonATPase subunit of the 19S regulator and is phosphorylated by AMP-activated protein kinase.

Alternative Names :

proteasome 26S non-ATPase subunit 11, S9, Rpn6, p44.5, MGC3844

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Ogris E., et al. (1999) J. Biol. Chem. 274: 14382-14391.;; Gagnon S.N., et al. (2002) Biochem. J. 368: 263-271.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human PSMD11 (1-422aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of PSMD11 in HeLa cells. The cell was stained with ATGA0446 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PSMD11 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HeLa cell lysate;;Lane 2.: 293T cell lysate;;Lane 3.: U87-MG cell lysate;;Lane 4.: NIH-3T3 cell lysate;;Lane 5.: PC3 cell lysate;; Lane 6.: TF1 cell lysate;; Lane 7.: MCF7 cell lysate

Reacts With :

Human

Clone Number :

AT2C7

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Ubiquitination

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

O00231

Internal Search Terms :

PSMD11,ATGA0446-10ug,ATGA0446-20ug,ATGA0446-50ug,ATGA0446-100ug,ATGA0446-250ug,ATGA0446-500ug,ATGA0446-1mg,ATGA0446-10,ATGA0446-20,ATGA0446-50,ATGA0446-100,ATGA0446-250,ATGA0446-500,ATGA0446-1

References with this Product :

Fukuyama K, et al. Roasted Coffee Reduces β-Amyloid Production by Increasing Proteasomal β-Secretase Degradation in Human Neuroblastoma SH-SY5Y Cells. (Mol Nutr Food Res. 2018){https://pubmed.ncbi.nlm.nih.gov/30144352/}

more info or order :

NKMAX product webpage