Human PPCS antibody | NKMAX ATGA0443 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human PPCS antibody

catalog :

ATGA0443

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT10D11

reactivity :

human

application :

western blot, ELISA

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human PPCS antibody

Catalog number (SKU) :

ATGA0443

NCBI :

NP_078940

Description :

PPCS, also known as phosphopantothenate--cysteine ligase, catalyzes the first step in the biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5), which is an essential universal pathway in prokaryotes and eukaryotes. PPCS, one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine.

Alternative Names :

Phosphopantothenate--cysteine ligase, COAB, PPC synthetase

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Manoj N., et al. (2003) Structure. 11: 927-936. ;; Christina Spry., et al. (2009) J Biol Chem. 284: 24904-24913.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human PPCS (1-311aa) purified from E. coli

Applications :

ELISA,WB

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPCS antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: Jurkat cell lysate ;;Lane 2.: HepG2 cell lysate ;;Lane 3.: HeLa cell lysate ;;Lane 4.: A549 cell lysate ;;Lane 5.: MCF7 cell lysate ;;Lane 6.: SK-OV-3 cell lysate

Reacts With :

Human

Clone Number :

AT10D11

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q9HAB8

Internal Search Terms :

PPCS, Phosphopantothenate--cysteine ligase, COAB, PPC synthetase,ATGA0443-10ug,ATGA0443-20ug,ATGA0443-50ug,ATGA0443-100ug,ATGA0443-250ug,ATGA0443-500ug,ATGA0443-1mg,ATGA0443-10,ATGA0443-20,ATGA0443-50,ATGA0443-100,ATGA0443-250,ATGA0443-500,ATGA0443-1

more info or order :

NKMAX product webpage