Human NCS1 antibody | NKMAX ATGA0442 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human NCS1 antibody

catalog :

ATGA0442

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT11B8

reactivity :

human

application :

western blot, ELISA, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human NCS1 antibody

Catalog number (SKU) :

ATGA0442

NCBI :

NP_055101

Description :

NCS1 is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. This protein regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. It is associated with secretory granules and modulates synaptic transmission and synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene.

Alternative Names :

Neuronal calcium sensor 1, Frequenin homolog, FLuP, FREQ, Neuronal calcium sensor 1 Flup, Freq, frequenin (Drosophila) homolog, Frequenin-like protein, NCS1, Neuronal Calcium Sensor 1.

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Nakamura TY., et al (2001) Proc. Natl. Acad. Sci. u.S.A. 98: 12808-12813. ;; Bahi N., et al (2003) Hum. Mol. Genet. 12: 1415-1425.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human NCS1 (1-190aa) purified from E. coli

Applications :

ELISA, WB, FACS

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NCS1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: 293T cell lysate ;;Lane 2.: K562 cell lysate ;;Lane 3.: LnCap cell lysate ;;Lane 4.: HeLa cell lysate

Reacts With :

Human

Clone Number :

AT11B8

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of NCS1 in A549 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). Flow cytometry analysis of NCS1 in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). {2004010381410490.jpg}

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P62166

Internal Search Terms :

NCS1, Neuronal calcium sensor 1, Frequenin homolog, FLuP, FREQ, Neuronal calcium sensor 1 Flup, Freq, frequenin (Drosophila) homolog, Frequenin-like protein, NCS1, Neuronal Calcium Sensor 1.,ATGA0442-10ug,ATGA0442-20ug,ATGA0442-50ug,ATGA0442-100ug,ATGA0442-250ug,ATGA0442-500ug,ATGA0442-1mg,ATGA0442-10,ATGA0442-20,ATGA0442-50,ATGA0442-100,ATGA0442-250,ATGA0442-500,ATGA0442-1

more info or order :

NKMAX product webpage