Human EphA2 antibody | NKMAX ATGA0441 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human EphA2 antibody

catalog :

ATGA0441

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT66G9

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human EphA2 antibody

Catalog number (SKU) :

ATGA0441

NCBI :

NP_004422

Description :

EPHA2, also known as ephrin type-A receptor 2, is a member of the ephrin receptor subfamily of the protein-tyrosine kinase family. It is implicated as positional labels that may guide the development of neural topographic maps. It has also been found implicated in embryonic patterning, neuronal targeting, vascular development and adult neovascularization.

Alternative Names :

EPH receptor A2, Ephrin type-A receptor 2, EPHA2, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK, ARCC2, CTPA, CTPP1, CTRCT6

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Flanagan JG., et al. (1998) Annu Rev Neurosci. 21: 309-345. ;; Cheng N., et al. (2002) Cytokine Growth Factor Rev. 13: 75-85.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human EPHA2 (27-537aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of EPHA2 in HeLa cell line, stained monoclonal anti-human EPHA2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of EPHA2 in A431 cell line, stained monoclonal anti-human EPHA2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). {ATGA0441-IF2.jpg}

WB Comment and Pic :

The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EPHA2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1 : PC3 cell lysate Recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EPHA2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1 : Recombinant human EPHA2 extracellular domain ;;Lane 2 : Recombinant human EPHA2 cytoplasmic domain {ATGA0441-WB2.jpg}

Reacts With :

Human

Clone Number :

AT66G9

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis, and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P29317

Internal Search Terms :

EphA2, Ephrin type-A receptor 2, EPHA2, ARCC2, CTPA, CTPP1, CTRCT6, ECK, AW545284, EC 2.7.10.1, Eck ,Eph receptor A2, EPHA2, EPHA2_HUMAN, Ephrin receptor, Ephrin receptor EphA2, Ephrin type A receptor 2, Ephrin type-A receptor 2, Epithelial cell kinase, Epithelial cell receptor protein tyrosine kinase, Myk 2, Myk2, Sek 2Sek2, Tyrosine protein kinase receptor ECK, Tyrosine-protein kinase receptor ECK, Tyrosine-protein kinase receptor MPK-5, Tyrosine-protein kinase receptor SEK-2,ATGA0441-10ug,ATGA0441-20ug,ATGA0441-50ug,ATGA0441-100ug,ATGA0441-250ug,ATGA0441-500ug,ATGA0441-1mg,ATGA0441-10,ATGA0441-20,ATGA0441-50,ATGA0441-100,ATGA0441-250,ATGA0441-500,ATGA0441-1

more info or order :

NKMAX product webpage