Human beta 2-Microglobulin/B2M antibody | NKMAX ATGA0439 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human beta 2-Microglobulin/B2M antibody

catalog :

ATGA0439

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT101F10

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human beta 2-Microglobulin/B2M antibody

Catalog number (SKU) :

ATGA0439

NCBI :

NP_004039

Description :

Beta2 microglobulin, also known as B2M, is a component of MHC class I molecules, Involved in the presentation of peptide antigens to the immune system. B2M is a protein found on the surface of many cells and plentiful on the surface of white blood cells. Increased production or destruction of these cells causes B2M levels in the blood to increase. This increase is seen in people with cancers involving white blood cells, but it is particularly meaningful in people newly diagnosed with multiple myeloma. Multiple myeloma is a malignancy (cancer) of a certain kind of white blood cell, called a plasma cell. B2M Testing is done primarily when evaluating a person for certain kinds of cancer affecting white blood cells including chronic lymphocytic leukemia, non-Hodgkin's lymphoma, and multiple myeloma or kidney disease.

Alternative Names :

Beta-2-microglobulin, CDABP0092, HDCMA22P

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Huang WC., et al (2010) J Biol Chem. 285(11): 7947-56. ;; Morabito A., et al. (2009) Hum Immunol. 70(7): 492-5.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human B2M (21-119aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of B2M in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human B2M antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). {ATGA0439-IF1b.jpg}

WB Comment and Pic :

The lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human B2M antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1 : HeLa cell lysate ;;Lane 2 : U937 cell lysate

Reacts With :

Human

Clone Number :

AT101F10

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis. Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of B2M in A431 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). Flow cytometry analysis of B2M in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). {ATGA0439-FC2.jpg}

Antigen Species :

Human

Gene Category :

Cardiovascular

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P61769

Internal Search Terms :

b2-Microglobulin, Beta-2-microglobulin, CDABP0092, HDCMA22P,ATGA0439-10ug,ATGA0439-20ug,ATGA0439-50ug,ATGA0439-100ug,ATGA0439-250ug,ATGA0439-500ug,ATGA0439-1mg,ATGA0439-10,ATGA0439-20,ATGA0439-50,ATGA0439-100,ATGA0439-250,ATGA0439-500,ATGA0439-1

more info or order :

NKMAX product webpage