Human BAG2 antibody | NKMAX ATGA0438 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human BAG2 antibody

catalog :

ATGA0438

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT29E9

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human BAG2 antibody

Catalog number (SKU) :

ATGA0438

NCBI :

NP_004273

Description :

BCL2-associated athanogene 2, also known as BAG2, is a member of the Bag family of proteins. BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain. BAG2 is a major component of the HSC 70/CHIP chaperone-dependent ubiquitin ligase complex and acts to disrupt CHIP-mediated ubiquitylation.

Alternative Names :

BCL2-associated athanogene 2, BAG-2

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Takayama S., et al. (1999) J Biol Chem. 274: 781-786.;; Arndt V., et al. (2005) CHIP Mol Biol Cell. 16: 5891-5900.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human BAG2 (1-211aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of BAG2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BAG2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). {ATGA0438-IF1b.jpg}

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BAG2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: Jurkat cell lysate ;;Lane 2.: A549 cell lysate ;;Lane 3.: K562 cell lysate ;;Lane 4.: HepG2 cell lysate ;;Lane 5.: HeLa cell lysate

Reacts With :

Human

Clone Number :

AT29E9

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Apoptosis

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

O95816

Internal Search Terms :

BAG2, BCL2-associated athanogene 2, BAG-2,ATGA0438-10ug,ATGA0438-20ug,ATGA0438-50ug,ATGA0438-100ug,ATGA0438-250ug,ATGA0438-500ug,ATGA0438-1mg,ATGA0438-10,ATGA0438-20,ATGA0438-50,ATGA0438-100,ATGA0438-250,ATGA0438-500,ATGA0438-1

more info or order :

NKMAX product webpage