Human FKBP22/FKBP14 antibody | NKMAX ATGA0436 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human FKBP22/FKBP14 antibody

catalog :

ATGA0436

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT18E2

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human FKBP22/FKBP14 antibody

Catalog number (SKU) :

ATGA0436

NCBI :

NP_060416

Description :

FKBP14, also known as 22 kDa FK506-binding protein, is an enzyme that accelerates the folding of proteins during protein synthesis. This protein contains two EF-hand domains and one PPIase FKBP-type domain. Truncation of the amino-terminus of FKBP14 greatly reduces peptidyl prolyl cis-trans isomerase activity, therefore suggesting that the PPIase FKBP-type domain must be located at the N-terminus.

Alternative Names :

22 kDa FK506 binding protein, FK506 binding protein 14 (22 kDa), FKBP22, Peptidyl prolyl cis trans isomerase, Peptidyl-prolyl cis-trans isomerase FKBP14, PPIase, Rotamase

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Tremmel D., et al. (2007) J Mol Biol. 369(1): 55-68. ;; Budiman C., et al. (2009) FEBS J. 276(15): 4091-101.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human FKBP14 (20-211aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of FKBP14 in HeLa cells. The cell was stained with ATGA0436 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FKBP14 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1. : HeLa cell lysate The cell lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FKBP14 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1. : 293T cell lysate ;;Lane 2. : FKBP14 Transfected 293T cell lysate {ATGA0436-WB2.jpg}

Reacts With :

Human

Clone Number :

AT18E2

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Development

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q9NWM8

Internal Search Terms :

22 kDa FK506 binding protein, FK506 binding protein 14, FK506 binding protein 14 (22 kDa), FKBP 22, FKBP22, Peptidyl prolyl cis trans isomerase, Peptidyl-prolyl cis-trans isomerase FKBP14, PPIase, Rotamase, FKBP14, FKBP 14, ATGA0436-10ug, ATGA0436-20ug, ATGA0436-50ug, ATGA0436-100ug, ATGA0436-250ug, ATGA0436-500ug, ATGA0436-1mg, ATGA0436-010, ATGA0436-020, ATGA0436-050, ATGA0436-100, ATGA0436-250, ATGA0436-500, ATGA0436-01M

more info or order :

NKMAX product webpage