Human S100A11 antibody | NKMAX ATGA0434 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human S100A11 antibody

catalog :

ATGA0434

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT20D11

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human S100A11 antibody

Catalog number (SKU) :

ATGA0434

NCBI :

NP_005611

Description :

S100A11 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. This protein may function in motility, invasion, and tubulin polymerization.

Alternative Names :

S100 calcium binding protein A11, MLN70, S100C

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Sakaguchi M., et al. (2003) J Cell Biol. 163: 825-35.;; Settmacher U., et al. (2005) Int. J. Oncol. 28: 195-200.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human S100A11 (1-105aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of S100A11 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human S100A11 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human S100A11 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1. : HeLa cell lysate ;;Lane 2. : MCF7 cell lysate ;;Lane 3. : PC3 cell lysate ;;Lane 4. : THP1 cell lysate ;;Lane 5. : U87MG cell lysate ;;Lane 6. : A427 cell lysate ;;Lane 7. : SK-OV-3 cell lysate ;;Lane 8. : A549 cell lysate

Reacts With :

Human

Clone Number :

AT20D11

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of S100a11 in HeLa cells. The cell was stained with ATGA0434 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray). Flow cytometry analysis of S100a11 in A549 cells. The cell was stained with ATGA0434 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray). {2004010373111810.jpg}

Antigen Species :

Human

Gene Category :

Cell Cycle

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P31949

Internal Search Terms :

S100A11, S100 calcium binding protein A11, MLN70, S100C,ATGA0434-10ug,ATGA0434-20ug,ATGA0434-50ug,ATGA0434-100ug,ATGA0434-250ug,ATGA0434-500ug,ATGA0434-1mg,ATGA0434-10,ATGA0434-20,ATGA0434-50,ATGA0434-100,ATGA0434-250,ATGA0434-500,ATGA0434-1

more info or order :

NKMAX product webpage