Human Inorganic Pyrophosphatase/PPA1 antibody | NKMAX ATGA0433 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Inorganic Pyrophosphatase/PPA1 antibody

catalog :

ATGA0433

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT4G4

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Inorganic Pyrophosphatase/PPA1 antibody

Catalog number (SKU) :

ATGA0433

NCBI :

NP_066952

Description :

PPA1 (Pyrophosphatase) belongs to the PPase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. The hydrolysis of inorganic pyrophosphate (PPi) to two phosphate ions is utilized in many biochemical pathways to render reactions effectively irreversible. Inorganic pyrophosphatase catalyzes this hydrolysis reaction in the early steps of lipid degradation, a prominent example of this phenomenon. By promoting the rapid hydrolysis of pyrophosphate (PPi), Inorganic pyrophosphatase provides the driving force for the activation of fatty acids destined for oxidation.

Alternative Names :

Inorganic pyrophosphatase, IOPPP, PP, PP1, SID6-8061

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Carman GM, et al., (2006) Trends Biochem. Sci. 31(12): 694-9. ;; Usui Y, et al., (2010) J. Dent. Res. 89(5): 504-9.

Abs Host :

Mouse

Isotype :

IgG2b Lambda

Immunogen :

Recombinant human PPA1 (1-289aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of Pyrophosphatase/PPA1 in HeLa cells. The cell was stained with ATGA0433 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPA1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1. : HeLa cell lysate ;;Lane 2. : A431 cell lysate ;;Lane 3. : 293T cell lysate ;;Lane 4. : HepG2 cell lysate ;;Lane 5. : U87MG cell lysate ;;Lane 6. : PC3 cell lysate ;;Lane 7. : WiDr cell lysate

Reacts With :

Human

Clone Number :

AT4G4

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of Pyrophosphatase/PPA1 in HeLa cells. The cell was stained with ATGA0433 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q15181

Internal Search Terms :

Inorganic Pyrophosphatase, PPA1, Inorganic pyrophosphatase, IOPPP, PP, PP1, SID6-8061,ATGA0433-10ug,ATGA0433-20ug,ATGA0433-50ug,ATGA0433-100ug,ATGA0433-250ug,ATGA0433-500ug,ATGA0433-1mg,ATGA0433-10,ATGA0433-20,ATGA0433-50,ATGA0433-100,ATGA0433-250,ATGA0433-500,ATGA0433-1

more info or order :

NKMAX product webpage