Human DECR1 antibody | NKMAX ATGA0431 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human DECR1 antibody

catalog :

ATGA0431

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT3B2

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human DECR1 antibody

Catalog number (SKU) :

ATGA0431

NCBI :

NP_001350

Description :

DECR1 is a mitochondrial protein that exists as a homotetramer and belongs to the family of short-chain dehydrogenases/reductases. It functions as an auxiliary enzyme of beta-oxidation andt participates in the metabolism of unsaturated fatty enoyl-CoA esters. Specifically, DECR1 uses NADP+ to catalyze the reduction of 2, 4-dienoyl-CoA to yield trans-3-enoyl-CoA, which can then be used as an intermediate in the Krebs cycle. Additionally, DECR1 is thought to function as a tumor suppressor, possibly downregulating the expression of Neu and slowing the rate of tumorigenesis.

Alternative Names :

24-dienoyl-CoA reductase mitochondrial, 2,4-dienoyl-CoA reductase, mitochondrial, DECR, NADPH, SDR18C1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Koivuranta KT., et al. (1994) Biochem J. 304(3): 787-92.;; Roe CR., et al. (1990) J Clin Invest. 85(5): 1703-7.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human DECR1 (35-335aa) purified from E. coli

Applications :

ELISA,WB,FACS,ICC/IF

IF Comment And Pic :

ICC/IF analysis of DECR1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human DECR1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DECR1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: PC3 cell lysate ;;Lane 2.: HepG2 cell lysate

Reacts With :

Human

Clone Number :

AT3B2

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of DECR1 in Hep3B cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q16698

Internal Search Terms :

DECR1, 24-dienoyl-CoA reductase mitochondrial, 2,4-dienoyl-CoA reductase, mitochondrial, DECR, NADPH, SDR18C1,ATGA0431-10ug,ATGA0431-20ug,ATGA0431-50ug,ATGA0431-100ug,ATGA0431-250ug,ATGA0431-500ug,ATGA0431-1mg,ATGA0431-10,ATGA0431-20,ATGA0431-50,ATGA0431-100,ATGA0431-250,ATGA0431-500,ATGA0431-1

more info or order :

NKMAX product webpage