Human Thioredoxin-2 antibody | NKMAX ATGA0427 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Thioredoxin-2 antibody

catalog :

ATGA0427

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1B1

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Thioredoxin-2 antibody

Catalog number (SKU) :

ATGA0427

NCBI :

NP_036605

Description :

Thioredoxin (Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The Thioredoxin2 may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis.

Alternative Names :

MTRX, TRX2, MT-TRX, Thioredoxin2, mitochondrial thioredoxin, thioredoxin 2 precursor, Thioredoxin mitochondrial, TRX 2, TXN 2, TXN2, Thioredoxin-2

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Wang Z., et al. (2007) Rapid Commun Mass Spectrom. 21(22): 3658-66.;; Zhang H., et al. (2007) Arch Biochem biophys. 465(1): 119-26.;; Holmgren, A. (1979) Journal of Biological Chemistry. 254, 9627-9632.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human Thioredoxin2 (60-166aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of TXN2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TXN2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Thioredoxin2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1 : HepG2 cell lysate ;;Lane 2 : HeLa cell lysate ;;Lane 3 : Raji cell lysate ;;Lane 4 : K562 cell lysate ;;Lane 5 : Jurkat cell lysate ;;Lane 6 : A431 cell lysate ;;Lane 7 : MCF7 cell lysate ;;Lane 8 : THP1 cell lysate Recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Thioredoxin2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1 : Recombinant human Thioredoxin1 protein ;;Lane 2 : Recombinant human Thioredoxin2 protein {ATGA0427-WB2.jpg}

Reacts With :

Human

Clone Number :

AT1B1

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q99757

Internal Search Terms :

Thioredoxin2, MTRX, TRX2, MT-TRX, Thioredoxin2, mitochondrial thioredoxin, thioredoxin 2 precursor, Thioredoxin mitochondrial, TRX 2, TXN 2, TXN2, Thioredoxin-2,ATGA0427-10ug,ATGA0427-20ug,ATGA0427-50ug,ATGA0427-100ug,ATGA0427-250ug,ATGA0427-500ug,ATGA0427-1mg,ATGA0427-10,ATGA0427-20,ATGA0427-50,ATGA0427-100,ATGA0427-250,ATGA0427-500,ATGA0427-1

more info or order :

NKMAX product webpage