Human Creatine kinase B/CKB antibody | NKMAX ATGA0426 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Creatine kinase B/CKB antibody

catalog :

ATGA0426

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT8E4

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Creatine kinase B/CKB antibody

Catalog number (SKU) :

ATGA0426

NCBI :

NP_001814

Description :

CKB (Creatine kinase B-type) is a cytoplasmic enzyme involved in energy homeostasis. The protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. Creatine kinases provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems including muscle, tumor and cancer cells.

Alternative Names :

Creatine kinase B-type, Brain creatine kinase, Creatine kinase B chain, Creatine phosphokinase B-type, CPK-B, B-CK, CKBB

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Chang EJ., et al. (2008) Nat Med. 14(9): 966-72. ;; Debrincat MA., et al. (2007) J Biol Chem. 282(7): 4728-37.;;

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human CKB (1-381aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of CKB in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CKB antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CKB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: HeLa cell lysate ;;Lane 2.: 293T cell lysate ;;Lane 2.: K562 cell lysate ;;Lane 2.: LnCap cell lysate

Reacts With :

Human

Clone Number :

AT8E4

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of CKB in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Neuroscience

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P12277

Internal Search Terms :

Creatine Kinase BB, Creatine kinase B-type, B-CK, CKBB, Creatine kinase B-type, BCK,ATGA0426-10ug,ATGA0426-20ug,ATGA0426-50ug,ATGA0426-100ug,ATGA0426-250ug,ATGA0426-500ug,ATGA0426-1mg,ATGA0426-10,ATGA0426-20,ATGA0426-50,ATGA0426-100,ATGA0426-250,ATGA0426-500,ATGA0426-1

more info or order :

NKMAX product webpage