Human UDP-glucose dehydrogenase/UGDH antibody | NKMAX ATGA0425 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human UDP-glucose dehydrogenase/UGDH antibody

catalog :

ATGA0425

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2G11

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human UDP-glucose dehydrogenase/UGDH antibody

Catalog number (SKU) :

ATGA0425

NCBI :

NP_003350

Description :

UGDH is a member of the UDP-glucose/GDP-mannose dehydrogenase family and is a ubiquitously expressed protein most abundant in the liver. This protein converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis.

Alternative Names :

UDP-glucose 6-dehydrogenase, UDP-Glc dehydrogenase, UDP-GlcDH, UDPGDH

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Bontemps Y., et al. (2003) J Biol Chem. 278(24): 21566-75.;; Vatsyayan J., et al. (2006) Biosci Biotechnol Biochem. 70(2): 401-10.;; Egger S., et al. (2011) J Biol Chem. 286(27): 23877-87.;;

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human UGDH (1-494aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of UGDH in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human UGDH antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). {ATGA0425-IF1b.jpg}

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UGDH antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: HeLa cell lysate ;;Lane 2.: NIH-3T3 cell lysate ;;Lane 3.: HepG2 cell lysate ;;Lane 4.: A549 cell lysate ;;Lane 5.: MCF7 cell lysate

Reacts With :

Human

Clone Number :

AT2G11

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

O60701

Internal Search Terms :

UGDH, uDP-glucose dehydrogenase, GDH, uDP-GlcDH, uDPGDH, uGD, uDP-glucose dehydrogenase, uDP-glucose 6-dehydrogenase,ATGA0425-10ug,ATGA0425-20ug,ATGA0425-50ug,ATGA0425-100ug,ATGA0425-250ug,ATGA0425-500ug,ATGA0425-1mg,ATGA0425-10,ATGA0425-20,ATGA0425-50,ATGA0425-100,ATGA0425-250,ATGA0425-500,ATGA0425-1

more info or order :

NKMAX product webpage