Human Glutathione Synthetase/GSS antibody | NKMAX ATGA0422 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Glutathione Synthetase/GSS antibody

catalog :

ATGA0422

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT12F2

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Glutathione Synthetase/GSS antibody

Catalog number (SKU) :

ATGA0422

NCBI :

NP_000169

Description :

Glutathione synthetase, also known GSS, is the second enzyme in the glutathione biosynthesis pathway. It catalyses the condensation of gamma-glutamylcysteine and glycine, to form glutathione. Defects in GSS are the cause of glutathione synthetase deficiency (GSS deficiency) ; also known as 5-oxoprolinuria or pyroglutamic aciduria. It is a severe form characterized by an increased rate of hemolysis and defective function of the central nervous system.

Alternative Names :

Glutathione synthetase, GSHS, GSH synthetase

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Polekhina G., et al. (1999) EMBO J. 18: 3204-3213. ;; Huanq Z A., et al. (2000) Biochim Biophys Acta. 1493: 48-55.;;

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human GSS (1-474aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of GSS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GSS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of GSS in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GSS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). {ATGA0422-IF2.jpg}

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GSS antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: MDA-MB231 cell lysate ;;Lane 2.: WiDr cell lysate ;;Lane 3.: SW480 cell lysate ;;Lane 4.: A427 cell lysate ;;Lane 5.: MCF7 cell lysate ;;Lane 6.: 293T cell lysate ;;Lane 7.: HeLa cell lysate ;;Lane 8.: HT1080 cell lysate ;;Lane 9.: U87MG cell lysate ;;Lane 10.: A549 cell lysate

Reacts With :

Human

Clone Number :

AT12F2

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P48637

Internal Search Terms :

GSS, Glutathione synthetase, GSHS, GSH synthetase,ATGA0422-10ug,ATGA0422-20ug,ATGA0422-50ug,ATGA0422-100ug,ATGA0422-250ug,ATGA0422-500ug,ATGA0422-1mg,ATGA0422-10,ATGA0422-20,ATGA0422-50,ATGA0422-100,ATGA0422-250,ATGA0422-500,ATGA0422-1

more info or order :

NKMAX product webpage