Human Jak2 antibody | NKMAX ATGA0421 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Jak2 antibody

catalog :

ATGA0421

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT53B7

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Jak2 antibody

Catalog number (SKU) :

ATGA0421

NCBI :

NP_004963

Description :

JAK2 is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis.

Alternative Names :

Janus kinase 2, Janus kinase 2, Janus kinase 2 (a protein tyrosine kinase), JTK10

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Bole-Feycot C., et al. (1998) Endocr Rev. 19(3): 225-68. doi:10.1210/er.19.3.225.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human JAK2 (1014-1132aa) purified from E. coli

Applications :

ELISA,WB,FACS,ICC/IF

IF Comment And Pic :

ICC/IF analysis of JAK2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human JAK2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human JAK2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1. : K-562 cell lysate ;;Lane 2. : TF-1 cell lysate ;;Lane 3. : 293T cell lysate ;;Lane 4 : Jurkat cell lysate ;;Lane 5 : THP-1 cell lysate

Reacts With :

Human

Clone Number :

AT53B7

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of JAK2 in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Cell Cycle

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

O60674

Internal Search Terms :

Jak2, Janus kinase 2, Janus kinase 2, Janus kinase 2 (a protein tyrosine kinase), JTK10,ATGA0421-10ug,ATGA0421-20ug,ATGA0421-50ug,ATGA0421-100ug,ATGA0421-250ug,ATGA0421-500ug,ATGA0421-1mg,ATGA0421-10,ATGA0421-20,ATGA0421-50,ATGA0421-100,ATGA0421-250,ATGA0421-500,ATGA0421-1

more info or order :

NKMAX product webpage