Human CapG antibody | NKMAX ATGA0420 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human CapG antibody

catalog :

ATGA0420

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1D10

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human CapG antibody

Catalog number (SKU) :

ATGA0420

NCBI :

AAH00728

Description :

Macrophage-capping protein, also known as CAPG, belongs to the gelsolin/villin family of actin-regulatory proteins. CAPG is a calcium-sensitive DNA-binding protein that plays a role in macrophage function. It is expressed in macrophages and macrophage-like cells and can localize both to the nucleus and the cytoplasm. This protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments.

Alternative Names :

Macrophage-capping protein, MCP, AFCP, Macrophage-capping protein Actin capping protein GCAP39, Actin regulatory protein CAP G, CAPG, Capping protein (actin filament) gelsolin like, Capping protein gelsolin like, gCap39, Gelsolin like capping protein, Macrophage capping protein, mbh1, Myc basic motif homolog 1.

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Kumar A., et al. (2015) Mol Cell Endocrinol. 404: 91-101.;; Dabiri G A., et al. (1992) J Biol Chem. 267: 16545-16552.;;

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human CAPG (1-348aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of CAPG in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CAPG antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). {ATGA0420-IF1b.jpg}

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAPG antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: HeLa cell lysate ;;Lane 2.: NIH3T3 cell lysate ;;Lane 3.: Mouse spleen tissue lysate ;;Lane 4.: THP-1 cell lysate ;;Lane 5.: Raw 264.7 cell lysate

Reacts With :

Human

Clone Number :

AT1D10

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Immunology

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P40121

Internal Search Terms :

CapG, Macrophage-capping protein, MCP, AFCP, Macrophage-capping protein Actin capping protein GCAP39, Actin regulatory protein CAP G, CAPG, Capping protein (actin filament) gelsolin like, Capping protein gelsolin like, gCap39, Gelsolin like capping protein, Macrophage capping protein, mbh1, Myc basic motif homolog 1.,ATGA0420-10ug,ATGA0420-20ug,ATGA0420-50ug,ATGA0420-100ug,ATGA0420-250ug,ATGA0420-500ug,ATGA0420-1mg,ATGA0420-10,ATGA0420-20,ATGA0420-50,ATGA0420-100,ATGA0420-250,ATGA0420-500,ATGA0420-1

more info or order :

NKMAX product webpage