Human 6-phosphogluconate Dehydrogenase/PGD antibody | NKMAX ATGA0419 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human 6-phosphogluconate Dehydrogenase/PGD antibody

catalog :

ATGA0419

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT46B3

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human 6-phosphogluconate Dehydrogenase/PGD antibody

Catalog number (SKU) :

ATGA0419

NCBI :

NP_002622

Description :

PGD (Phosphogluconate dehydrogenas), also known as 6PGD, is a 483 amino acid enzyme that is involved in the pentose phosphate shunt. Pentose is required for nucleic acid biosynthesis and the pentose phosphate cycle is a major source of NADPH. PGD deficiency increases the level of erythrocyte pyruvate kinase (PK) activity and reduces glutathione synthetase (GSH), resulting in hemolysis. Defects in PGD are generally asymptomatic and are inherited in an autosomal dominant fashion.

Alternative Names :

6-phosphogluconate dehydrogenase, Decarboxylating, PGDH, Phosphogluconate dehydrogenase, 6PGD

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Le TD., et al. (2010) Proc Natl Acad Sci U S A. 107(7): 3198-203.;; Tagen M., et al. (2009) J Immunol. 183(10): 6313-9;;

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human PGD (1-483aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of 6-phosphogluconate dehydrogenase/PGD1 in Hep3B cells. The cell was stained with ATGA0419 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGD antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: HeLa cell lysate ;;Lane 2.: MCF7 cell lysate ;;Lane 3.: 293T cell lysate ;;Lane 4.: A549 cell lysate ;;Lane 5.: Jurkat cell lysate

Reacts With :

Human

Clone Number :

AT46B3

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P52209

Internal Search Terms :

6-phosphogluconate dehydrogenase, PGD, Phosphogluconate dehydrogenase, 6PGD, Phosphogluconate dehydrogenase 0610042A05Rik, Au019875, C78335, 6 phosphogluconate dehydrogenase, decarboxylating, OTTMuSP00000011754, RP23-249G3.4,ATGA0419-10ug,ATGA0419-20ug,ATGA0419-50ug,ATGA0419-100ug,ATGA0419-250ug,ATGA0419-500ug,ATGA0419-1mg,ATGA0419-10,ATGA0419-20,ATGA0419-50,ATGA0419-100,ATGA0419-250,ATGA0419-500,ATGA0419-1

more info or order :

NKMAX product webpage