Human p27/Kip1 antibody | NKMAX ATGA0417 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human p27/Kip1 antibody

catalog :

ATGA0417

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT7B9

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human p27/Kip1 antibody

Catalog number (SKU) :

ATGA0417

NCBI :

NP_004055

Description :

The cyclin-dependent kinase inhibitor p27Kip1 plays an important role in cell cycle regulation. The cyclin-dependent kinase-inhibitory activity of p27Kip1 is regulated by changes in its concentration and its subcellular localization. Cyclin-dependent kinase inhibitor p27 (kip1) is a putative tumor suppressor for human cancer.

Alternative Names :

Cyclin-dependent kinase inhibitor 1B, CDKN4, KIP1, MEN1B, MEN4, P27KIP1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Fujita N., et al. (2003) J Biol Chem. 278(49): 49254-60.;; Viglietto G., et al. (2002) Nat Med. 8(10): 1136-44.;;

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human CDKN1b (1-198aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of CDKN1b in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CDKN1b antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). {ATGA0417-IF1b.jpg}

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CDKN1b antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1 : MCF-7 cell lysate ;;Lane 2 : NIH/3T3 cell lysate

Reacts With :

Human

Clone Number :

AT7B9

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Cell Cycle

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P46527

Internal Search Terms :

p27, Kip1, Cyclin-dependent kinase inhibitor 1B, CDKN4, KIP1, MEN1B, MEN4, P27KIP1,ATGA0417-10ug,ATGA0417-20ug,ATGA0417-50ug,ATGA0417-100ug,ATGA0417-250ug,ATGA0417-500ug,ATGA0417-1mg,ATGA0417-10,ATGA0417-20,ATGA0417-50,ATGA0417-100,ATGA0417-250,ATGA0417-500,ATGA0417-1

more info or order :

NKMAX product webpage