Human ASS1 antibody | NKMAX ATGA0416 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human ASS1 antibody

catalog :

ATGA0416

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1G11

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human ASS1 antibody

Catalog number (SKU) :

ATGA0416

NCBI :

NP_000041

Description :

ASS1 (argininosuccinate synthase or citrulline-aspartate ligase) belongs to the argininosuccinate synthase family. ASS1 is a urea cycle enzyme that is essential in the conversion of nitrogen from ammonia and aspartate to urea. A deficiency of ASS1 causes citrullinemia (CTLN1), an autosomal recessive disease which is characterized by severe vomiting spells and mental retardation. Cells deficient in ASS1 must absorb the arginine they need for growth from circulating blood.

Alternative Names :

Argininosuccinate synthase, ASS, CTLN1, Argininosuccinate synthase

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Chalikiopoulou C., et al. (2016) Pharmacogenomics. (4): 393-403.;; Rabinovich S., et al. (2015) Nature. 527(7578): 379-83.;; Walts AE., et al. (2015) J Cancer Res Clin Oncol. 141(8): 1363-9.;;

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human ASS1 (1-412aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of ASS1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ASS1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). {ATGA0416-IF1b.jpg}

WB Comment and Pic :

The MCF-7 cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ASS1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Reacts With :

Human

Clone Number :

AT1G11

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P00966

Internal Search Terms :

ASS1, Argininosuccinate synthase, ASS, CTLN1, Argininosuccinate synthase,ATGA0416-10ug,ATGA0416-20ug,ATGA0416-50ug,ATGA0416-100ug,ATGA0416-250ug,ATGA0416-500ug,ATGA0416-1mg,ATGA0416-10,ATGA0416-20,ATGA0416-50,ATGA0416-100,ATGA0416-250,ATGA0416-500,ATGA0416-1

more info or order :

NKMAX product webpage