Human CPOX antibody | NKMAX ATGA0414 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human CPOX antibody

catalog :

ATGA0414

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT36B10

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human CPOX antibody

Catalog number (SKU) :

ATGA0414

NCBI :

NP_000088

Description :

Coproporphyrinogen-III oxidase, mitochondrial precursor, also known as CPOX, is a 454 amino acid mitochondrial enzyme that is localized to the inner membrane space of erythrocytes. It participates in the sixth step of heme biosynthesis by catalyzing the formation of protoporphyrinogen IX from coproporphyrinogen III. Mutations in human CPOX gene predict the clinical outcome of the disease, with either hepatic hereditary coproporphyria or hematological manifestations of erythropoietic harderoporphyria.

Alternative Names :

Coproporphyrinogen-III oxidase mitochondrial precursor, Coproporphyrinogen-III oxidase, mitochondrial precursor, CPO, CPX, HCP

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Kim DH., et al. (2013) J Biochem. 154(6): 551-9.;; Barbaro M., et al. (2012) Clin Genet. 81(3): 249-56.;; Rosipal R., et al. (1999) Hum Mutat. 13(1): 44-53.;;

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human CPOX (111-454aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of CPOX in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CPOX antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CPOX antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: Recombinant protein CPOX ;;Lane 2.: 293T cell lysate ;;Lane 3.: HepG2 cell lysate ;;Lane 4.: A549 cell lysate ;;Lane 5.: Jurkat cell lysate ;;Lane 6.: K562 cell lysate ;;Lane 7.: LnCaP cell lysate

Reacts With :

Human

Clone Number :

AT36B10

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P36551

Internal Search Terms :

CPOX, Coproporphyrinogen-III oxidase mitochondrial precursor, Coproporphyrinogen-III oxidase, mitochondrial precursor, CPO, CPX, HCP,ATGA0414-10ug,ATGA0414-20ug,ATGA0414-50ug,ATGA0414-100ug,ATGA0414-250ug,ATGA0414-500ug,ATGA0414-1mg,ATGA0414-10,ATGA0414-20,ATGA0414-50,ATGA0414-100,ATGA0414-250,ATGA0414-500,ATGA0414-1

more info or order :

NKMAX product webpage