Human SHMT1 antibody | NKMAX ATGA0413 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human SHMT1 antibody

catalog :

ATGA0413

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT26E5

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human SHMT1 antibody

Catalog number (SKU) :

ATGA0413

NCBI :

AAH07979

Description :

Serine hydroxymethyltransferase 1, also known as SHMT1, belongs to the SHMT family. This protein is cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5 10-methylene tetrahydrofolate. Also, SHMT1 preferentially supplies one-carbon units for thymidylate biosynthesis, depletes methylenetetrahydrofolate pools for S-adenosylmethionine (SAM) synthesis by synthesizing serine, sequesters 5-methyltetrahydrofolate and inhibits.

Alternative Names :

Serine hydroxymethyltransferase 1, CSHMT, SHMT

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Garrow TA., et al. (1993) J Biol Chem. 268(16): 11910-6.;; Perry C., et al. (2005) J Biol Chem. 280(1): 396-400. ;; Pinthong C., et al. (2014) FEBS J. 281(11): 2570-83.;;

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human SHMT1 (1-483aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of SHMT1 in Hep3B cells. The cell was stained with ATGA0413 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SHMT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: A549 cell lysate ;;Lane 2.: HeLa cell lysate;; Lane 3.: NIH-3T3 cell lysate ;;Lane 4.: LnCaP cell lysate

Reacts With :

Human

Clone Number :

AT26E5

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Metabolism

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P34896

Internal Search Terms :

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more info or order :

NKMAX product webpage