Human AIF/AIFM1 antibody | NKMAX ATGA0411 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human AIF/AIFM1 antibody

catalog :

ATGA0411

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT22E9

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human AIF/AIFM1 antibody

Catalog number (SKU) :

ATGA0411

NCBI :

NP_665811

Description :

Apoptosis-inducing factor 1, also known as AIFM1, is a mitochondrial protein that translocates to the nucleus upon induction of apoptosis. AIFM1 has been shown to cause DNA fragmentation and chromatin condensation and to induce the release of cytochrome c and caspase-9 from mitochondria. Bcl-2 overexpression has been shown to prevent the release of AIFM1 from mitochondria, but not to block its apoptogenic activity. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy.

Alternative Names :

Apoptosis-inducing factor 1 mitochondrial isoform 2 precursor, AIF, CMTX4, COWCK, COXPD6, PDCD8

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Tang Y., et al. (2013) J Proteomics. 91C: 200-209.;; Kim TW., et al. (2013) Cell Death Dis. 4: e919.;;

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human AIFM1 (98-609aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of AIFM1 in Hep3B cells. The cell was stained with ATGA0411 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AIFM1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;; Lane 1. : Jurkat cell lysate;; Lane 2. : HeLa cell lysate;; Lane 3. : Hep3B cell lysate;; Lane 4. : Raji cell lysate;; Lane 5. : K562 cell lysate;; Lane 6. : MCF7 cell lysate;; Lane 7. : CTLL2 cell lysate The tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AIFM1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;; Lane 1. : Mouse heart tissue lysate;; Lane 2. : Mouse liver tissue lysate {ATGA0411-WB2.jpg}

Reacts With :

Human

Clone Number :

AT22E9

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Apoptosis

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

O95831

Internal Search Terms :

AIF, AIFM1, Apoptosis-inducing factor 1, mitochondrial isoform 2 precursor, Apoptosis-inducing factor 1 mitochondrial isoform 2 precursor, CMTX4, COWCK, COXPD6, PDCD8, ATGA0411-10ug, ATGA0411-20ug, ATGA0411-50ug, ATGA0411-100ug, ATGA0411-250ug, ATGA0411-500ug, ATGA0411-1mg, ATGA0411-010, ATGA0411-020, ATGA0411-050, ATGA0411-100, ATGA0411-250, ATGA0411-500, ATGA0411-01M

more info or order :

NKMAX product webpage