Human GRP58/PDIA3 antibody | NKMAX ATGA0410 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human GRP58/PDIA3 antibody

catalog :

ATGA0410

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT9E9

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human GRP58/PDIA3 antibody

Catalog number (SKU) :

ATGA0410

NCBI :

NP_005304

Description :

PDIA3, also known as protein disulfide-isomerase A3, is a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. This protein has protein disulfide isomerase activity. PDIA3 is also part of the major histocompatibility complex (MHC) class I peptide-loading complex (TAP1), which is essential for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface.

Alternative Names :

Protein disulfide isomerase family A member 3, Glucose regulated protein 58kDa, GPR58, Protein disulfide isomerase-associated 3, 58 kDa microsomal protein, P58, ERp61, Endoplasmic reticulum resident protein 57, ER protein 57, ERp57, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 60, ER protein 60, ERp60, GRP57, PI-PLC, HsT17083

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Huppa JB., Ploegh HL. (1998) Cell. 92: 145-8.;; Ellgaard L., Ruddock LW. (2005) EMBO Rep. 6: 28-32.;; Jessop CE., et al. (2007) EMBO J. 26: 28-40.;;

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human PDIA3 (25-505aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of PDIA3 in Hep3B cells. The cell was stained with ATGA0410 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDIA3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1. : HeLa cell lysate ;;Lane 2. : HepG2 cell lysate;; Lane 3. : NIH3T3 cell lysate ;;Lane 4. : Raw 264.7 cell lysate;; Lane 5. : LNCaP cell lysate

Reacts With :

Human

Clone Number :

AT9E9

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P30101

Internal Search Terms :

ERp57, PDIA3,ATGA0410-10ug,ATGA0410-20ug,ATGA0410-50ug,ATGA0410-100ug,ATGA0410-250ug,ATGA0410-500ug,ATGA0410-1mg,ATGA0410-10,ATGA0410-20,ATGA0410-50,ATGA0410-100,ATGA0410-250,ATGA0410-500,ATGA0410-1

more info or order :

NKMAX product webpage