Human Isocitrate Dehydrogenase 1/IDH1 antibody | NKMAX ATGA0409 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Isocitrate Dehydrogenase 1/IDH1 antibody

catalog :

ATGA0409

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT25H10

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Isocitrate Dehydrogenase 1/IDH1 antibody

Catalog number (SKU) :

ATGA0409

NCBI :

NP_005887

Description :

IDH1, also known as isocitrate dehydrogenase (IDHC) cytoplasmic enzyme, belongs to the isocitrate and isopropylmalate dehydrogenases family. This protein catalyzes the third step of the citric acid cycle, which involves the oxidative decarboxylation of isocitrate, forming alpha-ketoglutarate and CO2 in a two-step reaction. The first step involves the oxidation of isocitrate to the intermediate oxalosuccinate, while the second step involves the production of alpha-ketoglutarate. IDH1 is expressed in a wide range of species and also in organisms that lack a complete citric acid cycle.

Alternative Names :

Isocitrate dehydrogenase [NADP] cytoplasmic, IDCD, IDH, IDP, IDPC, PICD

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Bleeker FE., et al. (2009) Hum Mutat. 30(1): 7-11.;; Zhao S., et al. (2009) Science. 324(5924): 261-5.;;

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human IDH1 (1-414aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of IDH1 in Hep3B cells. The cell was stained with ATGA0409 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IDH1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1 : HepG2 cell lysate;; Lane 2 : A549 cell lysate ;;Lane 3 : HeLa cell lysate;; Lane 4 : NIH3T3 cell lysate;; Lane 5 : Jurkat cell lysate;; Lane 6 : K562 cell lysate;; Lane 7 : 293T cell lysate;; Lane 8 : MCF7 cell lysate;; Lane 9 : LnCaP cell lysate

Reacts With :

Human

Clone Number :

AT25H10

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Metabolism

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

O75874

Internal Search Terms :

Isocitrate Dehydrogenase1, IDH1,ATGA0409-10ug,ATGA0409-20ug,ATGA0409-50ug,ATGA0409-100ug,ATGA0409-250ug,ATGA0409-500ug,ATGA0409-1mg,ATGA0409-10,ATGA0409-20,ATGA0409-50,ATGA0409-100,ATGA0409-250,ATGA0409-500,ATGA0409-1

more info or order :

NKMAX product webpage