Human SGTA antibody | NKMAX ATGA0408 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human SGTA antibody

catalog :

ATGA0408

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT19E8

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human SGTA antibody

Catalog number (SKU) :

ATGA0408

NCBI :

NP_003012

Description :

SGTA, also known as SGT or hSGT, is a ubiquitously expressed protein that contains three TPR protein-protein interaction repeats. This protein is believed to function as a component of the androgen receptor (AR) -chaperone-cochaperone complex, acting as a cochaperone involved in androgen signaling. In addition, SGTA functions as a cochaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity. On the basis of its role in apoptosis and androgen signaling, SGTA is a potential candidate for PCOS (polycystic ovary syndrome), a disorder characterized by androgen excess, obesity and menstrual disturbances.

Alternative Names :

Small glutamine-rich tetratricopeptide repeat-containing protein alpha, alphaSGT, hSGT, SGT, Small glutamine-rich tetratricopeptide repeat-containing protein alpha Small glutamine rich tetratricopeptide repeat TPR containing alpha, uBP, Vpu binding protein

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Andreyeva A., et al. (2010) PLoS One. 5: e12018.;; Cziepluch C., et al. (1998) J Virol. 72(5): 4149-56.;;

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human SGTA (1-313aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of SGTA in Hep3B cells. The cell was stained with ATGA0408 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SGTA antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: HeLa cell lysate;; Lane 2.: K562 cell lysate ;;Lane 3.: A549 cell lysate;; Lane 4.: MCF7 cell lysate;; Lane 5.: LNCaP cell lysate;; Lane 6.: 293T cell lysate

Reacts With :

Human

Clone Number :

AT19E8

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Apoptosis

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

O43765

Internal Search Terms :

SGTA,ATGA0408-10ug,ATGA0408-20ug,ATGA0408-50ug,ATGA0408-100ug,ATGA0408-250ug,ATGA0408-500ug,ATGA0408-1mg,ATGA0408-10,ATGA0408-20,ATGA0408-50,ATGA0408-100,ATGA0408-250,ATGA0408-500,ATGA0408-1

more info or order :

NKMAX product webpage