Human PGM1 antibody | NKMAX ATGA0406 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human PGM1 antibody

catalog :

ATGA0406

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT84G2

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human PGM1 antibody

Catalog number (SKU) :

ATGA0406

NCBI :

NP_002624

Description :

PGM1 is an evolutionarily conserved enzyme that regulates one of the most important metabolic carbohydrate trafficking points in prokaryotic and eukaryotic organisms, catalyzing the bi-directional interconversion of glucose 1-phosphate (G-1-P) and glucose 6-phosphate (G-6-P). In one direction, G-1-P produced from sucrose catabolism is converted to G-6-P, the first intermediate in glycolysis. In the other direction, conversion of G-6-P to G-1-P generates a substrate for synthesis of UDP-glucose, which is required for synthesis of a variety of cellular constituents, including cell wall polymers and glycoproteins. PGM1 has been used extensively as a genetic marker for isozyme polymorphism among humans. PGM is known to be post-translationally modified by cytoplasmic glycosylation that does not seem to regulate its enzymatic activity but rather is implicated in the localization of the protein. Glucose 1, 6 bisphosphate (Glc-1, 6-P2), a powerful regulator of carbohydrate metabolism, has been demonstrated to be a potent activator of PGM. PGM1 is also modified by phosphorylation on Ser108 as part of its catalytic mechanism. This is shown to be performed by Pak1, a previously identified signaling kinase.

Alternative Names :

Phosphoglucomutase-1 isoform 1, CDG1T, GSD14

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Boros LG., et al. (2002) Pancreas. 24(1): 26-33.;; Dey NB., et al. (1994) The Journal of Biological Chemistry. 269(43): 27143-8.;; Gururaj A., et al. (2004) Oncogene. 23(49): 8118-27.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human PGM1 (1-562aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of PGM1 in HeLa cells. The cell was stained with ATGA0406 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGM1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1. : 293T cell lysate;; Lane 2. : HepG2 cell lysate ;; Lane 3. : NIH/3T3 cell lysate;; Lane 4. : Jurkat cell lysate;; Lane 5. : HeLa cell lysate;; Lane 6. : U87MG cell lysate ;;

Reacts With :

Human

Clone Number :

AT84G2

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Metabolism

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P36871

Internal Search Terms :

PGM1,ATGA0406-10ug,ATGA0406-20ug,ATGA0406-50ug,ATGA0406-100ug,ATGA0406-250ug,ATGA0406-500ug,ATGA0406-1mg,ATGA0406-10,ATGA0406-20,ATGA0406-50,ATGA0406-100,ATGA0406-250,ATGA0406-500,ATGA0406-1

more info or order :

NKMAX product webpage