Human SOD1/Cu-Zn SOD antibody | NKMAX ATGA0405 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human SOD1/Cu-Zn SOD antibody

catalog :

ATGA0405

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT6F1

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human SOD1/Cu-Zn SOD antibody

Catalog number (SKU) :

ATGA0405

NCBI :

NP_000445

Description :

Cu/Zn superoxide dismutase (SOD1), is a major antioxidant enzyme that catalyzes the conversion of superoxide anion to hydrogen peroxide and molecular oxygen. SOD1 facilitates the dismutation of oxygen radicals to hydrogen peroxide and also catalyzes pro-oxidant reactions, which include the peroxidase activity and hydroxyl radical generating activity. Also over 100 distinct SOD1 inherited mutations have been identified in the familial form of amyotrophic lateral sclerosis (ALS), a progressive degenerative disease of motor neurons. The mechanism by which mutant SOD1 induces the neurodegeneration observed in ALS is still unclear.

Alternative Names :

Superoxide dismutase 1 soluble, ALS, SOD, ALS1, IPOA, Cu-Zn superoxide dismutase, Superoxide dismutase 1 soluble, SOD1, Superoxide dismutase 1, soluble ALS 1, Amyotrophic lateral sclerosis 1 Amyotrophic lateral sclerosis 1 adult, Cu/Zn SOD, Cu/Zn superoxide dismutase, Homodimer, Indophenoloxidase A, Mn superoxide dismutase, SOD 1, SOD soluble, SOD2, SODC, Superoxide dismutase 1 soluble, Superoxide dismutase Cu Zn, Superoxide dismutase cystolic

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Deng HX., et al. (1993) Science. 261(5124): 1047-51.;; Valentine JS., et al. (2003) Proc Natl Acad Sci USA. 100(7): 3617-22.;; Rosen DR., et al. (1993) Nature. 362(6415): 59-62.;;

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human SOD1 (1-154aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF,FACS

IF Comment And Pic :

ICC/IF analysis of SOD1 in Hep3B cells. The cell was stained with ATGA0405 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SOD1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1. : A549 cell lysate;; Lane 2. : A431 cell lysate;; Lane 3. : 293T cell lysate;; Lane 4. : PC3 cell lysate;; Lane 5. : U87MG cell lysate;; The recombinant proteins (20ng) of SOD two isoform were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SOD1 (1:1000).;; Lane 1 : Recombinant protein SOD1;; Lane 2 : Recombinant protein SOD2 ;;{ATGA0405-WB2.jpg}

Reacts With :

Human

Clone Number :

AT6F1

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of SOD1 in Hep3B cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P00441

Internal Search Terms :

SOD1, Cu-Zn SOD,ATGA0405-10ug,ATGA0405-20ug,ATGA0405-50ug,ATGA0405-100ug,ATGA0405-250ug,ATGA0405-500ug,ATGA0405-1mg,ATGA0405-10,ATGA0405-20,ATGA0405-50,ATGA0405-100,ATGA0405-250,ATGA0405-500,ATGA0405-1

more info or order :

NKMAX product webpage