Human UDP-galactose-4-epimerase/GALE antibody | NKMAX ATGA0404 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human UDP-galactose-4-epimerase/GALE antibody

catalog :

ATGA0404

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT6G10

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human UDP-galactose-4-epimerase/GALE antibody

Catalog number (SKU) :

ATGA0404

NCBI :

NP_000394

Description :

The enzyme UDP-glucose 4-epimerase, also known as UDP-galactose 4-epimerase or GALE, is a homodimeric epimerase found in bacterial, fungal, plant, and mammalian cells. This enzyme performs the final step in the Leloir pathway of galactose metabolism, catalyzing the reversible conversion of UDP-galactose to UDP-glucose. GALE tightly binds nicotinamide adenine dinucleotide (NAD+), a co-factor required for catalytic activity. Additionally, human and some bacterial GALE isoforms reversibly catalyze the formation of UDP-N-acetylgalactosamine (UDP-GalNAc) from UDP-N-acetylglucosamine (UDP-GlcNAc) in the presence of NAD+, an initial step in glycoprotein or glycolipid synthesis.

Alternative Names :

UDP-glucose 4-epimerase, Galactowaldenase, UDP-N-acetylgalactosamine 4-epimerase, UDP-GalNAc 4-epimerase, UDP-N-acetylglucosamine 4-epimerase, UDP-GlcNAc 4-epimerase, UDP-galactose 4-epimerase, short chain dehydrogenase/reductase family 1E, member 1, SDR1E1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Holden HM., et al. (2003) J. Biol. Chem. 278(45): 43885-8.;; Liu Y., et al. (1996) Biochemistry. 35(23): 7615-20.;; Thoden Jb., et al. (2001) J. Biol. Chem. 276(18): 15131-6.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human GALE (1-348aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of GALE in Hep3B cells. The cell was stained with ATGA0404 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GALE antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;; Lane 1. : MCF7 cell lysate ;; Lane 2. : Jurkat cell lysate ;; Lane 3. : A431 cell lysate ;;Lane 4. : A549 cell lysate ;;Lane 5. : HeLa cell lysate;; Lane 6. : HepG2 cell lysate

Reacts With :

Human

Clone Number :

AT6G10

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Metabolism

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q14376

Internal Search Terms :

GALE,ATGA0404-10ug,ATGA0404-20ug,ATGA0404-50ug,ATGA0404-100ug,ATGA0404-250ug,ATGA0404-500ug,ATGA0404-1mg,ATGA0404-10,ATGA0404-20,ATGA0404-50,ATGA0404-100,ATGA0404-250,ATGA0404-500,ATGA0404-1

more info or order :

NKMAX product webpage