Human VTA1 antibody | NKMAX ATGA0403 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human VTA1 antibody

catalog :

ATGA0403

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT14G10

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human VTA1 antibody

Catalog number (SKU) :

ATGA0403

NCBI :

NP_057569

Description :

The VTA1 protein is involved in the endosomal multivesicular bodies (MVB) pathway. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. Thought to be a cofactor of VPS4A/B, which catalyzes disassembles membrane-associated ESCRT-III assemblies. This protein is involved in the sorting and down-regulation of EGFR (By similarity) and involved in HIV-1 budding.

Alternative Names :

vacuolar protein sorting-associated protein VTA1 homolog, DRG-1, DRG1, LIP5, SBP1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Jun Shi., et al. (2001) Brain Res. 910(1-2): 29-37. ;; Fujita H., et al. (2004) J Cell Sci. 117(Pt 14): 2997-3009.;; Ward DM., et al. (2005) J Biol Chem. 280(11): 10548-55.;;

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human VTA1 (1-307aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF,FACS

IF Comment And Pic :

ICC/IF analysis of VTA1 in Hep3B cells. The cell was stained with ATGA0403 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VTA1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;; Lane 1.: HepG2 cell lysate;; Lane 2.: 293T cell lysate;; Lane 3.: HeLa cell lysate;; Lane 4.: MCF7 cell lysate;; Lane 5.: A549 cell lysate;; Lane 6.: Jurkat cell lysate;; Lane 7.: K562 cell lysate;; Lane 8.: LnCaP cell lysate;; Lane 9.: U937 cell lysate;; Lane 10.: A431 cell lysate

Reacts With :

Human

Clone Number :

AT14G10

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of VTA1 in Hep3B cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Virus

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q9NP79

Internal Search Terms :

VTA1,ATGA0403-10ug,ATGA0403-20ug,ATGA0403-50ug,ATGA0403-100ug,ATGA0403-250ug,ATGA0403-500ug,ATGA0403-1mg,ATGA0403-10,ATGA0403-20,ATGA0403-50,ATGA0403-100,ATGA0403-250,ATGA0403-500,ATGA0403-1

more info or order :

NKMAX product webpage