Human IMPA1/IMP1 antibody | NKMAX ATGA0402 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human IMPA1/IMP1 antibody

catalog :

ATGA0402

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2G5

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human IMPA1/IMP1 antibody

Catalog number (SKU) :

ATGA0402

NCBI :

NP_005527

Description :

IMPA1 (inositol monophosphatase1) is responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides. It plays a key role in the phosphatidylinositol signaling pathway by catalyzing the hydrolysis of inositol monophosphates. This protein has been identified as the pharmacological target for lithium action in the brain.

Alternative Names :

Inositol monophosphatase 1, IMPA, IMP, IMPase 1, Inositol monophosphatase 1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Ganzhorn AJ., et al. (1996) Biochemistry. 35(33): 10957-66.;; Parthasarathy R., et al. (1997) Brain Res. 778(1): 99-106.;; Ohnishi T., et al. (2007) J Biol Chem. 282(1): 637-46.;;

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human IMPA1 (1-277aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of IMPA1 in Hep3B cells. The cell was stained with ATGA0402 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue). ICC/IF analysis of IMPA1 in LNCap cells. The cell was stained with ATGA0402 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue). {2004010341156324.jpg}

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IMPA1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: Jurkat cell lysate ;;Lane 2.: Raji cell lysate ;;Lane 3.: NIH/3T3 cell lysate

Reacts With :

Human

Clone Number :

AT2G5

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of IMPA1 in LNCap cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Neuroscience

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P29218

Internal Search Terms :

IMPA1, IMP1,ATGA0402-10ug,ATGA0402-20ug,ATGA0402-50ug,ATGA0402-100ug,ATGA0402-250ug,ATGA0402-500ug,ATGA0402-1mg,ATGA0402-10,ATGA0402-20,ATGA0402-50,ATGA0402-100,ATGA0402-250,ATGA0402-500,ATGA0402-1

more info or order :

NKMAX product webpage