Human Adenylosuccinate Lyase/ADSL antibody | NKMAX ATGA0400 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Adenylosuccinate Lyase/ADSL antibody

catalog :

ATGA0400

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT16C10

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Adenylosuccinate Lyase/ADSL antibody

Catalog number (SKU) :

ATGA0400

NCBI :

NP_000017

Description :

Adenylosuccinate lyase, also known as ADSL, is an enzyme that converts adenylosuccinate to AMP and fumarate as part of the purine nucleotide cycle. Defects in ADSL are the cause of adenylosuccinase deficiency (ADSL deficiency). ADSL deficiency is an autosomal recessive disorder characterized by the accumulation in the body fluids of succinylaminoimidazole-carboxamide riboside (SAICA-riboside) and succinyladenosine (S-Ado).

Alternative Names :

Adenylosuccinate lyase, AMPS, ASASE, ASL

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Marie S., et al. (2002) Am J Hum Genet. 71: 14-21. ;; Kmoch S., et al. (2000) Hum Mal Genet. 9: 1501-1513.;;

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human ADSL(1-484aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of ADSL in Hep3B cells. The cell was stained with ATGA0400 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The Recombinant Human ADSL (20ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ADSL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;; Lane 1. : Recombinant Protein ;; Lane 2. : HeLa cell lysate ;; Lane 3. : 293T cell lysate ;;Lane 4. : Jurkat cell lysate ;;Lane 5. : HepG2 cell lysate ;; Lane 6. : A549 cell lysate ;; Lane 7. : MCF7 cell lysate

Reacts With :

Human

Clone Number :

AT16C10

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of ADSL in Hep3B cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Metabolism

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P30566

Internal Search Terms :

ADSL,ATGA0400-10ug,ATGA0400-20ug,ATGA0400-50ug,ATGA0400-100ug,ATGA0400-250ug,ATGA0400-500ug,ATGA0400-1mg,ATGA0400-10,ATGA0400-20,ATGA0400-50,ATGA0400-100,ATGA0400-250,ATGA0400-500,ATGA0400-1

more info or order :

NKMAX product webpage