Human PGAM2 antibody | NKMAX ATGA0399 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human PGAM2 antibody

catalog :

ATGA0399

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT5A7

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human PGAM2 antibody

Catalog number (SKU) :

ATGA0399

NCBI :

NP_000281

Description :

Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. Since both 3-PGA and 2-PGA are allosteric regulators of the pentose phosphate pathway (PPP) and glycine and serine synthesis pathways, respectively, PGAM2 may contribute to the biosynthesis of amino acids, 5-carbon sugar, and nucleotides precursors. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). Mutations in this gene cause muscle phosphoglycerate mutase deficiency, also known as glycogen storage disease X. PGAM2 is one of two PGAM subunits found in humans and is predominantly expressed in adult muscle.

Alternative Names :

Phosphoglycerate mutase 2, GSD10, PGAM-M, PGAMM

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Tsujino S., et al. (1986) The Journal of biological chemistry. 264(26): 15334-7.;; Xu Y., et al. (2014) Cancer research. 74(13): 3630-42.;; Tsujino S., et al. (1993) American journal of human genetics. 52(3): 472-7.;;

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human PGAM2 (1-253aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of PGAM2 in Hep3B cells. The cell was stained with ATGA0399 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The recombinant protein (50ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGAM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;; Lane 1. : Recombnant protein (50ng);; Lane 2. : HeLa cell lysate ;; Lane 3. : HepG2 cell lysate;; Lane 4. : 293T cell lysate ;; Lane 5. : Jurkat cell lysate;; Lane 6. : NIH3T3 cell lysate;; Lane 7. : A549 cell lysate;; Lane 8. : MCF7 cell lysate

Reacts With :

Human

Clone Number :

AT5A7

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P15259

Internal Search Terms :

PGAM2,ATGA0399-10ug,ATGA0399-20ug,ATGA0399-50ug,ATGA0399-100ug,ATGA0399-250ug,ATGA0399-500ug,ATGA0399-1mg,ATGA0399-10,ATGA0399-20,ATGA0399-50,ATGA0399-100,ATGA0399-250,ATGA0399-500,ATGA0399-1

more info or order :

NKMAX product webpage