Human EMAP-II/AIMP1 antibody | NKMAX ATGA0396 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human EMAP-II/AIMP1 antibody

catalog :

ATGA0396

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT6D10

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human EMAP-II/AIMP1 antibody

Catalog number (SKU) :

ATGA0396

NCBI :

NP_001135888

Description :

AIMP1, also known as p43, hasn't until recently been recognized for its prominent immunological functions. Together with other nonenzymatic mARS structural components AIMp2/38 and AIMp3/p18, it participates in the machinery responsible for cell-cycle control and tumor suppression.

Alternative Names :

Aminoacyl tRNA synthase complex-interacting multifunctional protein 1, EMAP2, EMAPII, p43, SCYE1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Liang D., et al. (2015) Int Rev Immunol. 34(5): 367-381.;; Hong SH., et al. (2015) Biomaterials. 44: 45-54.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human AIMP1 (1-336aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of AIMP1 in Hep3B cells. The cell was stained with ATGA0396 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AIMP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1. : HeLa cell lysate ;;Lane 2. : HepG2 cell lysate;; Lane 3. : Raji cell lysate ;;Lane 4. : Jurkat cell lysate ;;Lane 5. : A549 cell lysate ;;Lane 6. : HaCaT cell lysate

Reacts With :

Human

Clone Number :

AT6D10

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of AIMP1 in Hep3B cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Apoptosis

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q12904

Internal Search Terms :

EMAP-2, AIMP1,ATGA0396-10ug,ATGA0396-20ug,ATGA0396-50ug,ATGA0396-100ug,ATGA0396-250ug,ATGA0396-500ug,ATGA0396-1mg,ATGA0396-10,ATGA0396-20,ATGA0396-50,ATGA0396-100,ATGA0396-250,ATGA0396-500,ATGA0396-1

more info or order :

NKMAX product webpage