Human Myc tag antibody | NKMAX ATGA0395 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Myc tag antibody

catalog :

ATGA0395

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT19B4

reactivity :

human

application :

western blot, ELISA

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Myc tag antibody

Catalog number (SKU) :

ATGA0395

NCBI :

NP_002458

Description :

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope.

Alternative Names :

Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, MYC, BHLHE39, c-Myc, MRTL, MYCC

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Cotterman R., et al. (2008) Cancer Res. 68(23): 9654-9662.;; Finver SN., et al. (1988) P NATL ACAD SCI. USA 85(9): 3052-3056.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human C-Myc (410-419aa) with tag purified from E. coli

Applications :

ELISA, WB

WB Comment and Pic :

The recombinant human SNCA-Myc tag proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Myc tag antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1. : Anti-human Myc tag monoclonal antibody (1:1000) ;;Lane 2. : Anti-Human Myc tag monoclonal antibody (1:5000);; Lane 3. : Anti-Human Myc tag monoclonal antibody (1:10000) The recombinant proteins were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Myc tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;; Lane 1. : Recombinant Human SNCA-Myc tag (20ng) ;; Lane 2. : Recombinant Human Myc-GST tag (50ng) ;;Lane 3. : Recombinant Human s100A8-Myc tag (50ng) ;;Lane 4. : Recombinant Human CHI3L1-Myc tag (50ng) {ATGA0395-WB2.jpg}

Reacts With :

Human

Clone Number :

AT19B4

Ab Usage :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Fusion Tag & Detection

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P01106

Internal Search Terms :

Myc,ATGA0395-10ug,ATGA0395-20ug,ATGA0395-50ug,ATGA0395-100ug,ATGA0395-250ug,ATGA0395-500ug,ATGA0395-1mg,ATGA0395-10,ATGA0395-20,ATGA0395-50,ATGA0395-100,ATGA0395-250,ATGA0395-500,ATGA0395-1

more info or order :

NKMAX product webpage