GAPDH antibody | NKMAX ATGA0394 product information

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product summary

company name :

NKMAX

product type :

antibody

product name :

GAPDH antibody

catalog :

ATGA0394

quantity :

100 ul

price :

290 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT4E8

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

product information

Product Type :

Antibodies

Item Name :

GAPDH antibody

Catalog number (SKU) :

ATGA0394

NCBI :

NP_002037

Description :

Multiple roles for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) have been recently appreciated. GAPDH is found in the particulate fractions, such as the nucleus, the mitochondria, and the small vesicular fractions. GAPDH gene expression is specifically increased during programmed neuronal cell death. When cells are exposed to various stressors, dynamic subcellular re-distribution of GAPDH occurs. GAPDH is also involved in various diseases, especially neurodegenerative disorders and cancers. As a membrane protein, GAPDH functions in endocytosis in the cytoplasm, it is involved in the translational control of gene expression in the nucleus, it functions in nuclear tRNA export, in DNA replication, and in DNA repair.

Alternative Names :

Glyceraldehyde-3-phosphate dehydrogenase isoform 2, G3PD, GAPD, HEL-S-162eP

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Chuang DM., et al, (2005) Annu Rev Pharmacol Toxicol. 45:269-90. ;; Mazzola JL., et al, (2002) Neurotoxicology. 23(4-5):603-9. ;; Sirover MA. (1997) J Cell Biochem. 66(2):133-40.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

GAPDH from rabbit muscle

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of GAPDH in HeLa cells. The cell was stained with ATGA0394 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-GAPDH antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: HepG2 cell lysate;; Lane 2.: MCF7 cell lysate;; Lane 3.: Jurkat cell lysate;; Lane 4.: A549 cell lysate;; Lane 5.: K562 cell lysate;; Lane 6.: A431 cell lysate;; Lane 7.: LNCap cell lysate The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-GAPDH antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Ramos cell lysate {ATGA0394-WB2.jpg} The tissue lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-GAPDH antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: Mouse Brain tissue lysate;; Lane 2.: Mouse Spleen tissue lysate;; Lane 3.: Mouse Eye tissue lysate;; Lane 4.: Mouse Stomach tissue lysate;; Lane 5.: Mouse Lung tissue lysate;; Lane 6.: Mouse Muscle tissue lysate {ATGA0394-WB3.jpg} The 293T cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GAPDH. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Anti-GAPDH monoclonal antibody (1:100,000);;Lane 2.: Anti-GAPDH monoclonal antibody (1:50,000);;Lane 3.: Anti-GAPDH monoclonal antibody (1:10,000);;Lane 4.: Anti-GAPDH monoclonal antibody (1:1,000) {ATGA0394-WB4.jpg}

Clone Number :

AT4E8

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Neuroscience

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P04406

Internal Search Terms :

GAPDH,

Size 1 :

100 ul

Price 1 (USD) :

290 USD