Human AK2 antibody | NKMAX ATGA0393 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human AK2 antibody

catalog :

ATGA0393

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT7E7

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human AK2 antibody

Catalog number (SKU) :

ATGA0393

NCBI :

NP_001616

Description :

Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Mutations in this gene are the cause of reticular dysgenesis. Alternate splicing results in multiple transcript variants.

Alternative Names :

Adenylate kinase 2, Mitochondrial Adenylate kinase 2, ATP-AMP transphosphorylase 2, ATP:AMP phosphotransferase, Adenylate monophosphate kinase, ADK2

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Kim D Pruitt., et al. (2012) Nucleic Acids Res. 40(Database issue): D130-D135.;; Lagresle-Peyrou C., et al. (2009) Nat Genet. 41(1): 106-11.;; Lee Y., et al. (1998) J. Biochem. 123 (1): 47-54.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human AK2 (1-239aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of AK2 in HeLa cells. The cell was stained with ATGA0393 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: Kidney tissue lysate;; Lane 2.: Liver tissue lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;; Lane 1.: HepG2 cell lysate;; Lane 2.: NIH/3T3 cell lysate {ATGA0393-WB2.jpg}

Reacts With :

Human

Clone Number :

AT7E7

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Apoptosis

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P54819

Internal Search Terms :

Mitochondrial Adenylate kinase 2, EC2743, EC2.7.4.3, EC 2.7.4.3, D4Ertd220e, ATP-AMP transphosphorylase 2, ATP:AMP phosphotransferase, ATP AMP transphosphorylase 2, ATP AMP transphosphorylase, ATP AMP phosphotransferase, Ak-2, AK2, Ak 2, ADK2, ADK 2, Adenylate monophosphate kinase, Adenylate kinase2, Adenylate kinase isoenzyme 2, Adenylate kinase 2 mitochondrial isoform a, Adenylate kinase 2, ATGA0393-10ug, ATGA0393-20ug, ATGA0393-50ug, ATGA0393-100ug, ATGA0393-250ug, ATGA0393-500ug, ATGA0393-1mg, ATGA0393-010, ATGA0393-020, ATGA0393-050, ATGA0393-100, ATGA0393-250, ATGA0393-500, ATGA0393-01M

more info or order :

NKMAX product webpage