Human Nicotinamide N-Methyltransferase/NNMT antibody | NKMAX ATGA0392 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Nicotinamide N-Methyltransferase/NNMT antibody

catalog :

ATGA0392

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT11G11

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Nicotinamide N-Methyltransferase/NNMT antibody

Catalog number (SKU) :

ATGA0392

NCBI :

NP_006160

Description :

N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor.

Alternative Names :

Nicotinamide N-methyltransferase

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Aksoy S., et al. (1995) Genomics. 29(3): 555-61.;; Gerhard DS., et al. (2004) Genome Res. 14(10B): 2121-7.;; Riederer M., et al. (2009) Atherosclerosis. 204(2): 412-7.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human NNMT (1-264aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of NNMT in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NNMT antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NNMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: HeLa cell lysate ;;Lane 2.: A549 cell lysate

Reacts With :

Human

Clone Number :

AT11G11

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of NNMT in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P40261

Internal Search Terms :

Nicotinamide N-Methyltransferase, NNMT, Nicotinamide N-methyltransferase, NNMT, Nicotinamide N-methyltransferase EC 2.1.1.1 Nicotinamide N methyltransferase.,ATGA0392-10ug,ATGA0392-20ug,ATGA0392-50ug,ATGA0392-100ug,ATGA0392-250ug,ATGA0392-500ug,ATGA0392-1mg,ATGA0392-10,ATGA0392-20,ATGA0392-50,ATGA0392-100,ATGA0392-250,ATGA0392-500,ATGA0392-1

more info or order :

NKMAX product webpage