Human GPI antibody | NKMAX ATGA0390 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human GPI antibody

catalog :

ATGA0390

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT22G2

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human GPI antibody

Catalog number (SKU) :

ATGA0390

NCBI :

NP_000166

Description :

Glucose-6-phosphate isomerase (GPI), also known as phosphoglucose isomerase (PGI) or phosphohexose isomerase (PHI), is an enzyme. In the cytoplasm, it functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phosphate (G6P) and fructose-6-phosphate (F6P). Extracellularly, it functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. GPI is also referred to as autocrine motility factor (AMF) based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in GPI are the cause of nonspherocytic hemolytic anemia, and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants.

Alternative Names :

Glucose-6-phosphate isomerase, GPI, AMF, GNPI, NLK, PGI, PHI, SA36, EC 5.3.1.9, Neuroleukin, Phosphoglucose isomerase, Phosphohexose isomerase, SA 36, Sperm antigen 36, G6PI, Glucose-6-phosphate isomerase

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Cordeiro AT., et al. (2003) Biochimica et biophysica acta. 1645(2): 117-122.;; Kugler W., et al. (2000) Baillieres Best Pract Res Clin Haematol. 13(1): 89-101.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human GPI (1-558aa) purified from E. coli

Applications :

ELISA,WB,FACS,ICC/IF

IF Comment And Pic :

ICC/IF analysis of GPI in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GPI antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

WB Comment and Pic :

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GPI antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1. : HeLa cell lysate ;;Lane 2. : HepG2 cell lysate ;;Lane 3. : A549 cell lysate ;;Lane 4. : Jurkat cell lysate ;;Lane 5. : U87MG cell lysate ;;Lane 6. : Raji cell lysate ;;Lane 7. : MCF7 cell lysate

Reacts With :

Human

Clone Number :

AT22G2

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of GPI in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Neuroscience

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P06744

Internal Search Terms :

GPI, Glucose-6-phosphate isomerase, GPI, AMF, GNPI, NLK, PGI, PHI, SA36, EC 5.3.1.9, Neuroleukin, Phosphoglucose isomerase, Phosphohexose isomerase, SA 36, Sperm antigen 36, G6PI, Glucose-6-phosphate isomerase,ATGA0390-10ug,ATGA0390-20ug,ATGA0390-50ug,ATGA0390-100ug,ATGA0390-250ug,ATGA0390-500ug,ATGA0390-1mg,ATGA0390-10,ATGA0390-20,ATGA0390-50,ATGA0390-100,ATGA0390-250,ATGA0390-500,ATGA0390-1

more info or order :

NKMAX product webpage