Human Gankyrin/PSMD10 antibody | NKMAX ATGA0386 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Gankyrin/PSMD10 antibody

catalog :

ATGA0386

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1F4

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Gankyrin/PSMD10 antibody

Catalog number (SKU) :

ATGA0386

NCBI :

NP_002805

Description :

26S proteasome non-ATPase regulatory subunit 10 is an enzyme that in humans is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides.

Alternative Names :

proteasome 26S subunit non ATPase 10 (PSMD10) isoform 1, ankyrin repeat protein p28, Gankyrin, 26S proteasome non-ATPase regulatory subunit 10, 26S proteasome regulatory subunit p28, PSMD10.

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Hori T., et al. (1998) Gene. 216(1): 113-122.;; Kaneko T., et al. (2009) Cell. 137: 914-925.;; Wang J., et al. (2009) Cytogenet Genome Res. 125: 87-97.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human PSMD10 (1-226aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of PSMD10 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PSMD10 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

WB Comment and Pic :

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PSMD10 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1. : A549 cell lysate ;;Lane 2. : PC3 cell lysate ;;Lane 3. : K562 cell lysate ;;Lane 4. : HepG2 cell lysate ;;Lane 5. : HeLa cell lysate ;;Lane 6. : Jurkat cell lysate

Reacts With :

Human

Clone Number :

AT1F4

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Ubiquitination

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

O75832

Internal Search Terms :

PSMD10, proteasome 26S subunit non ATPase 10 (PSMD10) isoform 1, ankyrin repeat protein p28, Gankyrin, 26S proteasome non-ATPase regulatory subunit 10, 26S proteasome regulatory subunit p28, PSMD10.,ATGA0386-10ug,ATGA0386-20ug,ATGA0386-50ug,ATGA0386-100ug,ATGA0386-250ug,ATGA0386-500ug,ATGA0386-1mg,ATGA0386-10,ATGA0386-20,ATGA0386-50,ATGA0386-100,ATGA0386-250,ATGA0386-500,ATGA0386-1

more info or order :

NKMAX product webpage